Abstract
Objective: To determine the increase of tooth enamel surface hardness after application hydroxyapatite paste that was synthesized from blood cockle shells (Anadara granosa) as a remineralization agent. Methods: Laboratory experimental study using twenty-seven maxillary first premolar and randomly divided into 3 groups. All of the samples were immersed in the non-cola carbonated drink (2 min). Thereafter, samples in each group were treated (6 min) with application of blood cockle shells paste that has been synthesized (group 1), casein phosphopeptide-amorphous calcium phosphate paste (GC Tooth Mousse®) (group 2) as a positive control, and stored in saline solution (NaCl) (group 3) as a negative control. Vickers Hardness Number (VHN) measurement was performed at baseline, after immersing in non-cola carbonated drink and after completing of the respective treatment. Results: Immersion in non-cola carbonated drink reduced the enamel surface hardness significantly. Significant re-hardening after treated occurred in group 1 and 2 also baseline hardness of both groups were achieved. But statistically no significant differences between group 1 and 2 in re-hardening enamel surface hardness (final hardness-hardness after immersion). Conclusion: Application of blood cockle shells paste as a remineralization agent could increase tooth enamel surface hardness which is nearly the same effective as CPP-ACP paste.
Published Version
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