Abstract
Given the necessity for increased conservation of endangered birds such as the Crested ibis, it is crucial to identify the gender of individuals fro m species with similar external mo rphologies among females (ZW) and males(ZZ) to establish a self-sustainable population for restoration of the species. Moreover, raising young chicks during the juvenile period based on sex determination encourages familiarity, wh ich might increase mating success rates. Thus, this study was carried out to determine the sex of Crested ibis chicks using three primers designed to check their identity. A se t of poly- merase chain reaction(PCR) primers was used to amplify a 276 bp chro mo-helicase-DNA b inding protein region(CHD) on the Z chro mosome in both sexes, whereas the other set was used to amp lify a 169 bp female -specific CHD sequence on the W chromosome. The sequences have been submitted to NCBI(GenBank Accession Number: AB620020, AB620021). Th is method will enable researchers to determine the gender of Crested ibis more simp ly and quickly.
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