Abstract

A total of 120 laying hens were employed in this investigation, with 20 hens per treatment and 10 repetitions (2 hens each replicate). The experimental treatments included the following: T1: Control group, which received a basil diet without the addition; T2: 20 milligrams of vitamin E per kilogram of feed; T3: 20 milligrams of octacosanol per kilogram of feed; T4: 40 milligrams of octacosanol per kilogram of feed; T5: 20 milligrams/Kg (mixed octacosanol + vitamin E). The findings indicate that the treatments including the addition of octacosanol significantly increased the percentage of eggs produced, improved feed conversion ratio, and increased the eggs mass. Compared to the first, second, and third treatments, the MDA concentration significantly decreasing during the fourth, fifth, and sixth treatments. There were no significantly variations in glutathione levels. The concentration of the GOT enzyme also decreased significantly across all treatments as compared to the control group. When compared to the control treatment, the third treatment significantly improved the concentration of the GPT enzyme. Estrogen levels were not significantly affected by any of the treatments.

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