Abstract
Abstract—In recent years, the demand for technical ethanol has increased due to its use in the transport sector. Xylose is the major five-carbon sugar obtained as a result of lignocellulose hydrolysis; however, the industrial producer of alcohol S. cerevisiae yeast ferments exclusively hexoses. Based on a recombinant strain capable of xylose metabolism, the derivatives with increased expression of the ZNF1 gene and deletion of the SIP4 gene encoding transcription factors were constructed. It was found that overexpression of the ZNF1 gene did not affect the fermentation of glucose or xylose. The deletion of the SIP4 gene did not affect the fermentation of glucose but resulted in a 29% decrease in ethanol production during xylose fermentation in comparison with the parental strain.
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