The impact of legume and cereal cover crops on rhizosphere microbial communities of subsequent vegetable crops for contrasting crop decline

Abstract

Crop decline is a progressive soil health reduction commonly associated to the specialized cultivations. As a series of biotic factors are involved in this phenomenon, it can be controlled with essentially agronomic methods, the impact of winter cover crops on early growth at subsequent vegetable crops was investigated in a two-year field study. Barley and hairy vetch were incorporated into the soil following two mechanical terminations of cover crops (green manure and green mulching). Immediately after, tomato and zucchini seedlings were transplanted and grown for 28days. The above-ground biomass of the vegetables was taken as indicator of crop response to pre-plant treatments, whilst root colonizing fungi and rhizosphere bacteria were the two analyzed soil microbial components. Root-colonizing fungi were evaluated using culture-based methods, bacteria were analyzed by amplification of rhizosphere soil DNA with 16S rDNA, then processed with PCR-DGGE. Tomato and zucchini growth response after vetch was always significantly higher than after barley regardless of mechanical termination. Rhizosphere bacterial communities differed significantly between cover crops and this differences was maintained also in the subsequent vegetable crops. Root-colonizing fungi differed between barley and vetch, although they shared most species (70%) such as Pythium spp., binucleate Rhizoctonia AG-A and several Fusarium spp. The latter three fungal groups were also found most abundant in tomato and zucchini roots. Pathogenicity test showed that tomato was more susceptible than zucchini to Pythium, Rhizoctonia solani, Fusarium oxysporum and Fusarium spp. and that their relationship with vegetable plants varied from pathogenic to neutral, up to mutualistic in the case of Rhizoctonia AG-A. These findings indicate difficulty to identify specific biotic agents responsible of crop decline. Tomato and zucchini showed an undoubted growth improvement after legume; however, a certain specificity of Cylindrocarpon-like fungi and Phoma spp. to hairy vetch suggests that, when exploiting benefit of this cover crop for mitigating yield decline of vegetable crops, legume should alternate cereal and other botanically distant genera in rotation.