Abstract
Objective To investigate the impact of immediate graft rear of transected nerve root on expression of C-Jun and Bcl-2 mRNA in proximal neurons in young rots.Methods Twenty-four 18-day-old SD rats were divided into two groups.In the nerve root resection group a 0.3 cm segment of C5 nerve root was resected.In the graft repair group resection of a 0.3 cm segment of C5 nerve root was followed by sural nerve grafting.C5 level spihal cord and dorsal root ganglion were harvested 4 weeks postoperatively.Real time reverse transcription polymerase chain reaction(RT-PCR)was carried out to measure the expression of C-Jun and Bcl-2 mRNA in the proximal neurons.Differences between the two groups were compared. Results Compared with C5 resection group,C-Jun mRNA in the proximal motor and sensory neurons of C5 graft repair group was significantly lower while Bcl-2 mRNA was significantly higher. Conclusion The difference in apoptotie gene expression in proximal neurons is likely the molecular mechanism for the neuronal protective effect of nerve grafting in brachial plexus birth palsy. Key words: Brachial plexus; Neurons; Apoptosis; Real time quantitative RT-PCR
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
