Abstract
Massively Parallel Sequencing (MPS) enables simultaneous analysis of a much larger number of forensic markers compared to conventional capillary electrophoresis (CE) sizing-based genotyping. Since both methods rely on amplified STR fragments, stutter products will occur that can complicate the detection of minor contributors in mixed samples. Sequence variation observed in STRs and their flanking regions can help to distinguish genuine alleles from stutter artefacts and noise correction can be applied to filter stutter artefacts. Using data from 545 samples analysed with the Forenseq™ DNA Signature Prep kit, a training set was used to obtain FDSTools noise profiles and a test set to analyse the impact of FDSTools noise correction for different analysis thresholds. Noise correction enabled a substantial reduction of analysis thresholds with limited drop-in down to 1.5%. However, occasional noise outliers (after correction) of up to 5% of the corresponding allele do occur and may complicate analysis.
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