The Impact of Eruca sativa Seeds on Leydig's Cells Number and Hormonal Profile In Cadmium Exposed Rats

Abstract

This study was aimed to investigate the protective role of Eruca sativa seeds extract on the number of Leydig's cells and hormonal profile in cadmium chloride treated rats.Forty adult male rats were randomly divided to four equal group and handled as follows for 56 days : Control group administered tap water ; group G1 administered tap water containing 30 ppm/ L of cadmium chloride; group G2 received tap water containing 30 ppm/ L of cadmium chloride and administered 250 mg/Kg.B.W of ethanolic extract of Eruca sativa seeds ; group G3 administered 250 mg/Kg.B.W of ethanolic extract of Eruca sativa seeds only.Collecting of blood samples were obtained at 0, 28 and 56 days for estimation of serum testesterone (T) , follicule stimulating hormon( FSH) and leutinizing hormon (LH) concentrations. At the end of experiment body weight and testicular weight were measurment. Furthermore, histological sections of testes were prepared for counting of Leydigs cells. A significant decrease in serum testesterone (T) , follicule stimulating hormon( FSH) and leutinizing hormon (LH) concentrations and decrease in body weight to testes weight% in group G1 at concentration of CdCl2 30 ppm/ L, as well as, statistical analysis and examination of histological sections of testes revealed a significant decrease in the number of Leydig's cells. Whereas, The results pointed the beneficial effects of Eruca sativa seeds extract to improvement the previous parameters, against cadmium chloride (G2), through a significant increase in hormonal profile concentration and numbers of Leydigs cells parallel with alleviating the testicular toxicity induced by CdCl2.