The impact of collagen sponge on wound healing

Abstract

Objective To explore the effect of collagen sponge on wound healing and its mechanism of regulating the response of wound inflammation.Methods 24 rats were divided into the control group and the experimental group by even and odd numbers of cages,12 rats in each group.Collagen sponges were applied locally on the round mechanical full-thickness skin wounds and changed each other day as the experimental group,whereas the gauze full of saline were applied as the control group.The wound area of each wound was measured on the day of model made and the 3rd,7th,14th,28th day,thereafter,as well as the scores of inflammation response TNF-o and IL-6 levels in the wound tissues measured before and 3,7 days after model made.Results 7 days after treatment,the areas of the wound in the experimental group [(0.49 ± 0.08) cm2] was significantly smaller than that of the control group [(0.92 ± 0.11)cm2].The wounds in 2 groups were totally healed on the 28th day.On the 3rd,7th day after modeling,the score of inflammation response in the control group were about (4.51 ± 1.01) and (2.25 ± 0.62) respectively,whereas those of the experimental group were about (3.58 ± 0.51) and (1.66 ± 0.49) respectively.The differences of the two groups were statistically significant(t =2.85,2.59,all P ＜ 0.01).7 days after modeling,the amount of TNF-oin the tissues of the control group was (512 ±62)ng · L-1 · g-1,which was significantly higher than (434 ± 37) ng · L-1 · g-1 of the experimental group (t =3.74,P ＜ 0.05).The amount of IL-6 in the control group 3,7 days after modeling were (2 215 ±314)ng · L-1 · g-1 and (1 562 ± 174)ng · L-1 · g-1 respectively,which were significantly higher than (1672 ± 161)ng · L-1 · g-1 and (669 ±75)ng · L-1 · g-1 in the experimental group (t =5.33,16.33,all P ＜ 0.01).Conclusion Collagen sponge can intervene inflammatory reaction and promote the angiogenesis of the wound,promote wound healing. Key words: Collagen; Inflammation mediators; Wound healing