THE IMPACT OF CHOLECALCIFEROL ON THE REPRODUCTIVE SYSTEM FUNCTION IN RATS MALES WITH EXPERIMENTAL GONADOPATHY

Abstract

Actuality. Nowadays, the disturbances of men’s reproductive function are the important problem which often accompany sex glands diseases. During last years, scientific researchers are taking into consideration vitamin D with aim to create safe medicines or treatment schemes for therapy hypofertility developed due to gonadopathies. It is thought, Vitamin D may improve not only spermogramm, but, probably, acts on men’s reproductive ability by influencing fertility. The correction of reproductive disturbances requires the experimental grounding of vitamin D and its complex schemes using on the infertility experimental models. The aim of this investigation was to determine the condition of rat males’ reproductive system with experimental damage of testicles during correction by vitamin D3. Materials and methods. The correction by vitamin D3 (cholecalciferol) as a monotherapy or together with Tribestan® has been carried out in Vistar rats with experimental gonadopathy induced by Serotonin hydrochloride. The drug of comparison Tribestan® has been introduced per os in dose of 68 mg/kg body mass three days before Serotonin hydrochloride introduction; under the condition of serotonin obtaining (14 days) and during three days after its last injection once a day. Vitamin D3 has been given according to the same scheme in value of 0,5 ml in dose of 4000 IU (per os). The male fertility has been determined according to fertility and fecundity indices of rats’ females fertilized by experimental rats’ males. Due to results of mating with intact females the integral index of average realized fecundity (fertility) of rats’ males has been calculated. The researches of spermatogenesis has been carried out in epididymis’s suspension, the morphometric indices were studied on the histological sections. Results. Under the condition of serotonin induced gonadopathy the disturbance of all indices of fertility and reproductive function has been observed. The introduction of cholecalciferol as a monotherapy during three weeks has statistically increased the part of motile epididymal sperms relative to gonadopathy, but not reaching the intact animals level; the number of spermatogonia and index of spermatogenesis have been normalized. However, the vitamin D using hasn’t influenced general number and percentage of pathological forms of sperm cells in rats with modelled testicles damage; hasn’t improve males’ fertility. The introduction of vitamin D3 together with Tribestan to rats mails with serotonin induced testicles damage has positively affected all the spermogramm and gonads morphometric indices. The fecundity, as an embryonic losses level, has almost declined to Control rats’ values, whereas, the number of alive fetuses in thus group has been statistically higher than in groups with pathology as well as in those animals which obtained only cholecalciferol or referent medicine separately. The index of realized fecundity Fi has normalized in rats obtained vitamin D3 together with Tribestan. Fi in this group was higher than in other experimental animals. Conclusions. The using of cholecalciferol in complex schemes of treating of reproductive system disturbances due to gonads damage has led to improving of reproductive function and may be considered being pathogenetic therapy due to influence the different chains of reproductive health.