Abstract
Objective To observe the calcium-sensing receptor (CaSR) high pulmonary blood flow in rats with pulmonary hypertension pulmonary mitochondrial function,and to explore the mechanism of the formation of its high pulmonary blood flow in pulmonary hypertension.Methods Row left lung resection establish a rat model of pulmonary hypertension.27 rats were randomly divided into 3 groups (n =9),the control group,the surgery group,surgery calcium-sensing receptor blockers Calhex231 group.Rats mean pulmonary artery pressure were measured after feeding 35d detect right ventricular/body weight (right ventricle/body weight,RV/BW) and right ventricular/left ventricular interventricular septum (right ventricle/left and lodging to plus septum,RV/LV S) ratio.The test groups the rat pulmonary CaSR mRNA expression; mitochondrial ultrastructure; perspective electronmicroscope pulmonary the TdT-mediated dUTP nick end labeling (TUNEL) method statistics rats in each group rate of endothelial cell apoptosis.Results Rat pulmonary artery endothelial cells CaSR mRNA relative expression levels of 2-△△ct,the control group compared with the control group 0.0003 ±0.0001,0.0071 ±0.0041,the surgery group difference was statistically significant (P ＜ 0.05) ; surgery group pulmonary mitochondrial membrane swelling,mitochondrial activity decreased pulmonary endothelial cell apoptosis was significantly increased (P ＜ 0.05).The surgery Calhex231 rats pulmonary CaSRmRNA relative expression level of 0.0011 ±0.0003,compared with the surgery group decreased significantly (P ＜ 0.05),and reduce the swelling of the mitochondrial membrane,vitality has been restored,the pulmonary endothelial cell apoptosis rate was significantly lower (P ＜ 0.05).Conclusion The high pulmonary blood flow can cause the activation of rat pulmonary artery endothelial cells CaSR regulating mitochondrial pathway to induce apoptosis of pulmonary artery endothelial cells,causing pulmonary hypertension. Key words: Calcium-sensitive receptors; High pulmonary blood flow; Pulmonary hypertension; Smooth muscle
Published Version
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