Abstract
The stable and efficient operation of the activated sludge sequencing batch reactor (ASSBR) in heavy oil refineries has become an urgent necessity in wastewater biotreatment. Hence, we constructed a green and efficient solid bioaugmentation agent (SBA) to enhance the resistance of the reactor to loading shock. The impact of bioaugmentation on the performance and microbial community dynamics under three patterns of heavy oil refinery wastewater (HORW) loading shock (higher COD, higher toxicity, and higher flow rate) was investigated on an industrial-scale ASSBR. Results showed that the optimal SBA formulation was a ratio and addition of mixed bacteria Bacillus subtillis and Brucella sp., of 3:1 and 3.0%, respectively, and a glucose concentration of 5.0 mg/L. The shock resistance of ASSBR was gradually enhanced and normal performance was restored within 6–7 days by the addition of 0.2% SBA. Additionally, the removal efficiency of chemical oxygen demand and total nitrogen reached 86% and 55%, respectively. Furthermore, we found that Burkholderiaceae (12.9%) was replaced by Pseudomonadaceae (17.1%) in wastewater, and Lachnospiraceae (25.4%) in activated sludge was replaced by Prevotellaceae (35.3%), indicating that the impact of different shocks effectively accelerated the evolution of microbial communities and formed their own unique dominant bacterial families.
Highlights
IntroductionHeavy oil refinery wastewater (HORW) contains high amounts of recalcitrant and toxic organic pollutants, such as petroleum hydrocarbons, emulsified oil, sulfur compounds, and dissolved and dispersed oil [1]
This study aimed to investigate the effects of bioaugmentation on the performance and microbial community dynamics of an industrial-scale activated sludge sequencing batch reactor (ASSBR) under various loading shocks of heavy oil refinery wastewater (HORW)
This study mainly investigated the effect of different concentrations of carbon sources, BY2, and BN3 on the biodegradability of simulated HORW because glucose was added to the solid bioaugmentation agent (SBA) to accelerate the rapid growth and activity of BY2 and BN3 in the ASSBR
Summary
Heavy oil refinery wastewater (HORW) contains high amounts of recalcitrant and toxic organic pollutants, such as petroleum hydrocarbons, emulsified oil, sulfur compounds, and dissolved and dispersed oil [1]. Traditional treatment of heavy oil refinery wastewater is time-consuming and expensive. The concentration of these organic pollutants fluctuates significantly during daily operations. HORW has been recognized as one of the most refractory wastewaters [2]. It is very costly to treat HORW and very challenging to maintain the stability of the wastewater treatment systems
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