The IL-6-174C/G polymorphism analysis in Ukrainian residents as prospects for biomedical and pharmacogenetic uses

Abstract

It is reliably determined that the presence of the G allele in the 174C/G polymorphic region of the IL-6 gene promoter and a higher level of IL-6 are more commonly observed among patients suffering from various metabolic disorders and obesity, malignant tumors, type 2 diabetes, periodontitis, oxidative stress. Over time, muscle damage and inflammation processes develop. Aim. To study the frequency of the IL-6-174C/G single nucleotide polymorphism in the Ukrainian population. Materials and methods. Buccal swab samples for the DNA analysis were collected from 102 healthy volunteers (48 males, 54 females, Ukrainian residents, predominantly ethnical Ukrainians). Genotyping to determine the IL-6-174C/G polymorphism was performed on DNA samples from the buccal epithelium using the polymerase chain reaction followed by RELP. Control of the genotype distribution for compliance with the Hardy–Weinberg equilibrium was performed using the χ2 criterion. Results and discussion. The distribution of genotypes of the IL-6-174C/G polymorphism in the Ukrainian population samples was as follows: CC – 46 %, CG – 49 %, and GG – 5 % of residents. The frequency of the IL-6-174C/G polymorphism allele in the population was pС – 0.71 and qG – 0.29. The population structure did not deviate from the Hardy–Weinberg equilibrium since there was no difference between the theoretically expected and actual frequencies of three genotypes. Conclusion. The data obtained demonstrates the presence of the IL-6-174C/G polymorphism in the Ukrainian population.