The Identification and Verification of Key Long Noncoding RNAs in Ischemic Stroke

Houxi Xu,Wenguo Yang,Xinyue Jing,Yuzhu Ma,Xia Chen,Yaoyao Bian,Shuping Fu,Jialin Gu,Jinzhi Zhang,Shengfeng Lu,Vida Demarin

doi:10.1155/2020/2094320

Abstract

Stroke is a neurological disease with high rates of mortality and disability. The pathogenesis of stroke is acute focal injury of the central nervous system, leading to impaired neural function. Ischemic stroke accounts for the majority of cases. At present, the exact molecular mechanism of ischemic stroke remains unclear. Studies have shown that long noncoding RNAs (lncRNAs) have an important regulatory role in biological processes, participating in the regulation of transcription and affecting the processing and splicing of mRNAs. Abnormal lncRNA expression is associated with various diseases, including diseases of the nervous system. To identify and verify the key lncRNAs in ischemic stroke, we downloaded gene expression data from the National Center for Biotechnology Information Gene Expression Omnibus (NCBI GEO) and obtain differentially expressed lncRNAs, miRNAs, and mRNAs by bioinformatics analysis. Cytoscape was used to reconstruct a lncRNA-miRNA-mRNA network on the basis of the competitive endogenous RNA theory. We performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of the mRNAs regulated by lncRNAs in the lncRNA-miRNA-mRNA network. The resulting lncRNA-miRNA-mRNA network was composed of 91 lncRNA nodes, 70 mRNA nodes, 21 miRNA nodes, and 288 edges. GO analysis and KEGG pathway analysis have shown that 191 GO terms and 23 KEGG pathways were enriched. Finally, we found that four key lncRNAs were highly correlated with ischemic stroke and could be used as potential new targets for treatment.

Highlights

Stroke is considered the second leading cause of death after ischemic heart disease and the third leading cause of disability worldwide [1]
There have been many studies related to Long noncoding RNAs (lncRNAs), few studies have examined the key lncRNAs associated with ischemic stroke
We explored the key lncRNAs that influence the development of ischemic stroke from the perspective of epigenetics using bioinformatics methods

Summary

Introduction

Stroke is considered the second leading cause of death after ischemic heart disease and the third leading cause of disability worldwide [1]. The precise molecular mechanism of ischemic stroke is not yet known. There is an urgent need to clarify the etiological mechanism of ischemic stroke and to identify new biomarkers and therapeutic targets. A number of related studies have shown that the processes involved in the occurrence and development of various diseases are associated with the abnormal expression of lncRNAs. For example, high expression of lncSNHG15 can promote the proliferation of cancer cells in intestinal, breast, prostate, BioMed Research International and lung cancer [5,6,7,8]. There have been many studies related to lncRNAs, few studies have examined the key lncRNAs associated with ischemic stroke

Methods

Results

Discussion

Conclusion