Abstract
Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100%. The digestive tract has the most common Vibrio strains (V. alginolyticus [16] V. azureus [7] and V. fluvialis). Thereout, 25 of 30 strains of Vibrio contained 1 to 3 toxin genes, except V. parahaemolyticus. Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that number of separate polymorphic sites (S), total number of mutant sites (Eta), number of haplotype (h), haplotype diversity (Hd), average number of nucleotide differences (k), nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980 × 10–3 ± 0.003 (Pi), respectively (P < 0,05). The G + C content above 1434 sites positions of nucleotide sequences accounted for 0.542. The phylogenetic tree showed that these strains are divided into six groups. As observed, the appearance of isolated Vibrio on 3 organs of fish (S. ocellatus) hemorrhagic are V. azureus (27,67%), V. alginolyticus (50%), V. orientalis (6,67%) and V. fluvialis (16,67%). Through this result, we found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species.
Highlights
More than 100 Vibrio spp. have been reported and are predominantly associated with a variety of marine, estuarine, or other aquatic habitats (Janda 2015)
The results indicated that all Polymerase chain reaction (PCR) products of the 16S rRNA region in the 30 isolated bacterial strains based on medium Thiosulphate citrate bile salt sucrose (TCBS) showed a single band with a 100% amplification rate
The results showed that the nucleotide sequence 16S rRNA regions are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis and V. orientalis published in GenBank, ranging from 98.05 to 100%
Summary
More than 100 Vibrio spp. have been reported and are predominantly associated with a variety of marine, estuarine, or other aquatic habitats (Janda 2015). In recent years, red drum (S. ocellatus) mortalities have. Toxin gene neutrality was tested by three methods (Tajima’s D test- Methods for sequence analysis of candidate genes obtained; Fu and Li’s D* and F* test, Fu’s Fs—statistic P values being significant or not), (Tajima 1989), (Fu and Li 1993) and (Fu 1995), they indicated an excess of low frequency polymorphisms relating to expectation, evidence for a deficiency of alleles, as expected from a recent population bottleneck and the evolution of the studied 30 strains bacteria Vibrio, was balancing selection, sudden contraction, rare alleles appeared in populations with low frequency. The study aims to identify and determine toxin genes in Vibrio infected red drum; an understanding of Vibrio spp. infected on fish to cause Vibriosis in aquatic animals in brackish and marine water
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