基于mtCOII基因对山东省越冬代亚洲玉米螟不同种群的遗传结构分析

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 基于mtCOII基因对山东省越冬代亚洲玉米螟不同种群的遗传结构分析 DOI: 10.5846/stxb201204120521 作者: 作者单位: 山东省农业科学院植保所,山东省农业科学院植保所,青岛农业大学农学与植物保护学院,青岛农业大学农学与植物保护学院,青岛农业大学农学与植物保护学院 作者简介: 通讯作者: 中图分类号: 基金项目: 现代农业产业技术体系专项资金CARS-02;"泰山学者"建设工程专项 Genetic structure of the overwintering Asian corn borer,Ostrinia furnacalis (Guenée)collections in Shandong of China based on mtCOII gene sequences Author: Affiliation: SAAS,,,,QINGDAO AGRICULTURAL UNIVERSITY Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:亚洲玉米螟Ostrinia furnacalis(Guenée)是我国重要农业害虫。为了全面揭示亚洲玉米螟遗传分化以及山东省亚洲玉米螟不同种群的遗传结构,对山东省越冬代亚洲玉米螟不同种群mtCOII基因序列与来自GenBank的相关序列进行了遗传结构分析,并建立了鉴别不同遗传支系的PCR-RFLP方法。基于对340条mtCOII序列的分析结果表明:所有样本共获得62个单倍型,其中单倍型17(H17)广泛分布于各种群之间,有6个单倍体型为山东种群所特有;亚洲玉米螟分化为2个遗传支系(支系Ⅰ与支系Ⅱ);2个遗传支系均在山东省发现,但以支系I为主;亚洲玉米螟各单倍体型散布于山东省各地理种群中,缺乏明显的地理分布格局。山东亚洲玉米螟总体的单倍体型多样性指数Hd为0.695,种群内单倍型多样性指数在0.333-0.889之间;总体的核酸多样性指数π为0.00424,种群内核酸多样性指数在0.00061-0.00809之间。总群体的固定系数 Fst 为0.79421。AMOVA分析结果表明山东亚洲玉米螟的遗传分化主要来自于2个支系之间(79.42%)。构建的亚洲玉米螟2个支系鉴别方法为其生物学与生态学的进一步研究奠定了基础。 Abstract:Ostrinia furnacalis(Guenée), the Asian corn borer, is one of the most important agricultural pests in China. It can damage crops during different crop growth stages, and can affect the yield and quality of maize. In addition, the pest has a wide geographic distribution and host range. With global warming and the expansion of corn acreage in recent years, the occurrence of the pest and associated damages have been increasing. Studying the genetic structure of the Asian corn borer is necessary for understanding the pest's evolution and migration in Shandong, a critical corn production region in China. In order to reveal the genetic diversity and structure of O. furnacalis in Shandong, China, and throughout the world, we analyzed 340 mtCOII sequences of O. furnacalis obtained both from the present study and GenBank (updated to October 1st, 2011). Of the mtCOII sequences analyzed, 214 sequences were obtained from GenBank while 126 sequences were obtained during the present study. The mtCOII sequences were aligned using MEGA5.05 and were then checked for indels and numts. Using DnaSP 5.0, a set of genetic parameters for mtCOII were estimated including: the number of polymorphic (segregating) sites (S); the total number of mutations (η); the average number of nucleotide differences (K); the number of haplotypes (H); the haplotype diversity (Hd);the nucleotide diversity (π), defined as the average number of pairwise nucleotide differences per site; and the nucleotide diversity with Jukes and Cantor correction for different host collections from Shandong Province. The results revealed that there were a total of 62 haplotypes, among which the haplotype H17 was the most widely distributed. Using the 62 haplotypes, a phylogenetic tree was constructed with the maximum likehood (ML) method; two clades (Clade Ⅰ and Clade Ⅱ) were revealed. The populations from Shandong Province consisted of two clades, although Clade I was the dominant clade. The haplotypes from Shandong were distributed randomly among 17 populations with no obvious geographical pattern. The haplotype diversity (Hd) of the populations from Shandong ranged from 0.333 to 0.889, and the Hd of the entire populations in Shandong was 0.695. The nucleotide diversity (π) of the populations from Shandong ranged from 0.00061 to 0.00809 and the π of total populations was 0.00424. Analysis of molecular variance (AMOVA) showed that 79.42% of the total genetic variance was contributed by the inter-clade variation and only 20.58% contributed by the intra-clade variation. Finally, to rapidly differentiate the two clades, a PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism) method was developed. The PCR-RFLP method of differentiating the two clades within O. furnacalis will be helpful for future research on the biological and ecological differences between the two clades. Our work revealed that the incidence of Clade Ⅱ was less than Clade Ⅰ. This might suggest that they are different in biology, ecology and physiology. Such differences could affect the clade's geographic distributions and population diffusions. These studies' results serve as a guide for the sustainable control of the pest, however these issues need to be further studied. 参考文献 相似文献 引证文献