Abstract
Treatment of samples of live virus vaccines by electrophoresis in agar and agarose gels at both pH 6 and pH 8 with subsequent bio-autography using Bacillus subtilis enabled the antibiotic constituents of such vaccines to be analysed. The sensitivity of the method permitted the identification and assay of low levels of penicillin, streptomycin, neomycin, gentamicin and kanamycin which are commonly used in the manufacture of these products.
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