Abstract
Understanding the interaction mechanisms between xylan and xylan-degrading enzymes is beneficial to the efficient hydrolysis of xylan. Xylan from sugarcane bagasse (SB) was extracted and characterized. The effects of heat treatment and removal of side chains of SB xylan on the hydrolytic efficiency and synergistic action of endo-β-1,4-xylanases (HoXyn11A and AnXyn10C), β-xylosidases (AnXln3D), and α-l-arabinofuranosidases (AnAxh62A) were investigated. Results indicated that heat treatment of xylan can improve the hydrolytic efficiency of xylan-degrading enzymes, and it is essential for the efficient hydrolysis of xylan by HoXyn11A. The removal of arabinofuranosyl side chains of xylan by AnAxh62A before enzymatic hydrolysis reduced the hydrolytic efficiency of HoXyn11A and AnXyn10C on xylan. AnXyn10C was more efficient than HoXyn11A in hydrolysis of xylan, whereas HoXyn11A showed better synergistic action than AnXyn10C with AnAxh62A and AnXln3D in the hydrolysis of xylan. This study provides new insights on the enzymatic hydrolysis of SB into monosaccharides and xylo-oligosaccharides.
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