The hydrolysis of α‐N‐benzoyl‐L‐arginine ethyl ester by monolayers of papain and trypsin

Abstract

AbstractThe hydrolysis of α‐N‐Benzoyl‐L‐arginine ethyl ester (BAEE) as a substrate under monolayers of papain and trypsin at the air–water interface was studied using an automated Lauda film balance to characterize the monolayers and a small resin‐flask reactor with movable barriers for surface‐layer compression. The rates of reaction were followed by UV spectrophotometry. The hydrolysis rate increased with moderate increases in surface pressure and temperature.