Abstract
A high confidence set of proteins in urine from healthy donors is described as a reference urinary proteome.
Highlights
Urine is a desirable material for the diagnosis and classification of diseases because of the convenience of its collection in large amounts; all of the urinary proteome catalogs currently being generated have limitations in their depth and confidence of identification
Identification of urinary proteins Normal total protein concentration in urine is very low and usually does not exceed 10 mg/100 ml in any single specimen
Concentrated protein from single urine sample was separated by one-dimensional sodium dodecyl sulfate (SDS)polyacrylamide gel electrophoresis (PAGE) and reverse phase high-performance liquid chromatraphy (HPLC)
Summary
Urine is a desirable material for the diagnosis and classification of diseases because of the convenience of its collection in large amounts; all of the urinary proteome catalogs currently being generated have limitations in their depth and confidence of identification. Our laboratory has developed methods for the in-depth characterization of body fluids; these involve a linear ion trap-Fourier transform (LTQ-FT) and a linear ion trap-orbitrap (LTQ-Orbitrap) mass spectrometer. We applied these methods to the analysis of the human urinary proteome. Protein concentration in normal donor urine is very low (less than 100 mg/l when urine output is 1.5 l/day), and normal protein excretion is less than 150 mg/day. This is about a factor 1000 less compared with other body fluids such as plasma. Excretion of more than 150 mg/day protein is defined as proteinuria and is indicative of glomerular or reabsorption dysfunction
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