Abstract
Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow‐derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia‐like cells to use in cell‐based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR‐MP). STR‐MP single‐cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial‐specific genes present in development and CNS pathologies. STR‐MPs can be expanded and generate microglia‐like cells in vitro, which we name stromal microglia (STR‐M). STR‐M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia‐like precursors that can be used in patient‐centered fast derivative approaches.
Highlights
Microglia are generated from yolk sac erythromyeloid progenitors (EMPs) that populate the developing central nervous system (CNS) to
Current methods of generating induced pluripotent stem cells that subsequently can be differentiated to microglia-like cells are timeconsuming (≥4 months), have low efficiency, and are potentially associated with tumor risks when used for transplantation purposes.[10,11]
We demonstrate the presence of a nonhematopoietic cell population, named stromal microglial progenitors (STR-MPs), in the stromal compartment of the human bone marrow (hBM)
Summary
Microglia are generated from yolk sac erythromyeloid progenitors (EMPs) that populate the developing central nervous system (CNS) to.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call