The Hill Reaction of Red Kidney Bean Chloroplasts.

Abstract

The Hill reaction rates of red kidney bean chloroplasts prepared by usual procedures were found to be low compared to those of spinach. Active chloroplasts could be prepared only if Versene was added to the homogenizing buffer. Raising the pH of the grinding buffer to S or over could substitute in part for the presence of Versene. Once the leaves were ground, no further changes in chloroplast activity could be induced by reciprocation of the above treatment. Chloroplasts with low activity have apparently been damaged in a dark step, not in the light-dependent part of the Hill reaction. 8 references, 3 figures, 5 tables.