Abstract
The Hill reaction of chloroplasts was inhibited by washing them with 0.8 M Tris buffer. This inhibition was further promoted by adding ferricyanide in the washing medium. When a reducing reagent, such as the 2,6-dichlorophenol indophenol (DCPIP)-ascorbate system or the hydroquinone (HQJ-ascorbate system, had been added to the Tris buffer, Hill reaction activity was unaffected. Hill reaction activity of Tris-washed chloroplasts recovered up to 70% of the initial level by re-washing the chloroplasts with a preparation medium containing theabove reducing reagents. Photobleaching of carotenoid and chlorophyll is characteristic of Tris-washed chloroplasts. However, reactivated chloroplasts showed no photobleaching as in the case with intact chloroplasts.
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