Abstract
The staphylococcal biofilm-based infections of wounds still pose a significant therapeutical challenge. Treated improperly, they increase the risk of limb amputation or even death of the patient. The present algorithms of infected wound treatment include, among others, the application of antiseptic substances. In vitro wound biofilm models are applied in order to scrutinize their activity. In the present work, using a spectrum of techniques, we showed how the change of a single variable (medium composition) in the standard in vitro model translates not only to shift in staphylococcal biofilm features but also to the change of efficacy of clinically applied wound antimicrobials such as octenidine, polyhexamethylene biguanide, chlorhexidine, hypochlorite solutions, and locally applied gentamycin. The data presented in this study may be of a pivotal nature, taking into consideration the fact that results of in vitro analyses are frequently used to propagate application of specific antimicrobials in hospitals and ambulatory care units.
Highlights
The treatment of chronic wound infection is still considered a challenge for contemporary medicine and includes application of time-consuming algorithms involving actions performed by multidisciplinary medical team [1]
Results indicated that all strains were able to form biofilm in applied in vitro settings, but they showed significant correlation (p < 0.05) between level of biomass and metabolic activity for strains cultured in tryptic soy broth (TSB)+G and Dulbecco’s modified Eagle’s medium (DMEM), but not in TSB medium (Figure 1)
The determination coefficients indicated low curve fitting for tryptic soy soy broth withwith glucose (TSB+G) (R2 = 0.12) and medium for DMEM (R2 = 0.55)
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Chronic wound infection has a significant impact on a patient’s health, and on their social and economic status. Untreated or treated improperly, infected chronic wound is a factor significantly increasing risk of limb amputation or development of systemic, life-threatening inflammatory symptoms. The treatment of chronic wound infection is still considered a challenge for contemporary medicine and includes application of time-consuming algorithms involving actions performed by multidisciplinary medical team [1]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
