The Hidden Diversity of Diatrypaceous Fungi in China.

Xinlei Fan,Ning Jiang,Chengming Tian,Dongqin Dai,Rong Ma,Haiyan Zhu,Meng Pan,Nalin N Wijayawardene

doi:10.3389/fmicb.2021.646262

Xinlei Fan, Ning Jiang + Show 6 more

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https://doi.org/10.3389/fmicb.2021.646262

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Abstract

In this study, we investigated the diversity of diatrypaceous fungi from six regions in China based on morpho-molecular analyses of combined ITS and tub2 gene regions. We accept 23 genera in Diatrypaceae with 18 genera involved in the phylogram, and the other five genera are lacking living materials with sequences data. Eleven species included in four genera (viz. Allocryptovalsa, Diatrype, Diatrypella, and Eutypella) have been isolated from seven host species, of which nine novel species (viz. Allocryptovalsa castaneae, A. castaneicola, Diatrype betulae, D. castaneicola, D. quercicola, Diatrypella betulae, Da. betulicola, Da. hubeiensis, and Da. shennongensis), a known species of Diatrypella favacea, and a new record of Eutypella citricola from the host genus Morus are included. Current results show the high diversity of Diatrypaceae which are wood-inhabiting fungi in China.

Highlights

Diatrypaceae is an important family in Xylariales (Sordariomycetes, Ascomycota), containing many taxa with a worldwide distribution (Glawe and Jacobs, 1987; Mayorquin et al, 2016; Senwanna et al, 2017; Moyo et al, 2018a,b; Konta et al, 2020; Wijayawardene et al, 2020)
Several plant pathogens of Diatrypaceae were reported causing canker, dieback, and grapevine trunk diseases, e.g., Cryptosphaeria populina was linked to canker in Populus species (Glawe and Rogers, 1984); Cryptosphaeria pullmanensis caused canker disease in Populus alba and Salix alba (Ma et al, 2016); Cryptovalsa ampelina caused grapevine trunk disease on Vitis species (Luque et al, 2006); Eutypa lata was isolated from Prunus armeniaca and Vitis species with canker and dieback symptoms (Lardner et al, 2005); Eutypa leptoplaca contributed to the dieback of grapevines (Trouillas and Gubler, 2004; Catal et al, 2007); and Eutypella parasitica caused canker in Acer species (Rappaz, 1987)
The phylogenetic analysis combined internal transcribed spacer (ITS) and tub2 contained 146 ingroup strains with 1,175 characters including gaps (713 for ITS and 462 for tub2), of which 471 were constant, 191 variable characters were parsimony-uninformative, and 513 characters were variable and parsimony-informative

Summary

Introduction

Diatrypaceae is an important family in Xylariales (Sordariomycetes, Ascomycota), containing many taxa with a worldwide distribution (Glawe and Jacobs, 1987; Mayorquin et al, 2016; Senwanna et al, 2017; Moyo et al, 2018a,b; Konta et al, 2020; Wijayawardene et al, 2020). An endophyte of Picea abies, a gymnosperm host, was identified as an asexual morph of Diatrypaceae (Libertella sp.) by Caroll et al (1977). Few endophytes such as Diatrypella frostii and Peroneutypa scoparia were reported later (de Errasti et al, 2010; Vieira et al, 2011). Several plant pathogens of Diatrypaceae were reported causing canker, dieback, and grapevine trunk diseases, e.g., Cryptosphaeria populina was linked to canker in Populus species (Glawe and Rogers, 1984); Cryptosphaeria pullmanensis caused canker disease in Populus alba and Salix alba (Ma et al, 2016); Cryptovalsa ampelina caused grapevine trunk disease on Vitis species (Luque et al, 2006); Eutypa lata was isolated from Prunus armeniaca and Vitis species with canker and dieback symptoms (Lardner et al, 2005); Eutypa leptoplaca contributed to the dieback of grapevines (Trouillas and Gubler, 2004; Catal et al, 2007); and Eutypella parasitica caused canker in Acer species (Rappaz, 1987).

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