Abstract
AbstractObjective:To determine conversion formulas so that values from the HemoCue®hemoglobinometer (HbHQ) could be equated to hemoglobin concentrations measured by the gold‐standard cyanomethemoglobin (HbCY) method and used for estimation of packed cell volume (PCV) in cats.Study design:Prospective,in vitro.Animals:Twelve healthy, adult, client‐owned cats.Interventions:The PCV of 12 parent blood samples was manipulated between ∼3 and ∼80% by removing or adding autologous plasma. Hemoglobin was measured by the HbCYmethod at a university clinical pathology laboratory and by the azidemethemoglobin method in a HbHQ. PCV was determined by micro‐centrifugation.Measurements and main results:Hemoglobin varied from 1–26 g/dL. Repeated‐measures regression of HbCYon HbHQdemonstrated that theY‐intercept was not different from zero. When the regression was repeated, forcing the line through the origin the coefficient for converting HbHQto HbCYwas not significantly different from 1.0 (adjustedR2=99.7%). The conversion formula was HbCY=HbHQ. Using similar methods, the conversion formula for estimating PCV was PCV=3.1 HbHQ(adjustedR2=99.8%).Conclusion:Hemoglobin concentration measured by the HemoCue®was indistinguishable from the (HbCY) method in feline blood over a wide range of hemoglobin concentrations. This study demonstrates that in cats PCV=3.1HbHQ.Clinical relevance:The HemoCue®is useful for point‐of‐care hemoglobinometry and for estimating PCV in cats.
Published Version
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