The hematological changes associated with ovine subclinical mastitis.

Abstract

The aim of the present study was to investigate ovine subclinical mastitis and associated with the hematological changes were studied in awassi sheep flock consists of ninety ovine lactating (58 normal and 32 ewes subclinical mastitis) both groups aged 1.5-4 years in AL-Najaf province /Iraq. Milk samples were collected for California mastitis test (CMT) and blood samples were collected from jugular vein during the period February 2016 till April 2016.The results showed that the means ± SE in normal lactating and ewes subclinical mastitis were as follows; Packed cell volume (PCV) 31.9 ± 0.4 % and 25 ± 0.3 %, hemoglobin (Hb) 10.3 ± 0.16 g/dl and 7.3 ± 0.12 g/dl, red blood cell count (RBC) 10.2 ± 0.13 ×10⁶/μL and 8.9 ± 0.28 ×10⁶/μL, mean cell volume (MCV) 31.3 ± 0.36 fl and 30.9 ± 0.62 fl, mean cell hemoglobin (MCH) 10.1 ± 0.12 pg and 9.7 ± 0.21 pg, mean cell hemoglobin concentration (MCHC) 32.2 ± 0.18 g/dl and 31.2 ± 0.36 g/dl, erythrocyte sedimentation rate (ESR) 9.9 ± 0.46 mm/24 hrs and 17.9 ± 2.08 mm/24 hrs, Platelets 283.8 ± 16.6×10³/μL and 184.5 ± 8.3×10³/μL, white blood cell count (WBC) 10784 ± 268.3×10³/μL and 13956 ± 578.4×10³/μL, lymphocytes 54.3 ± 1.36 and 50.9 ± 2.51/μL, neutrophils 40.7 ± 1.41 and 52.8 ± 1.84/μL, monocytes 1.83 ± 0.16 and 3.0 ± 0.27/μL, eosinophil’s 2.84 ± 0.31 and 5.81 ± 0.76/μL and basophils 0.28 ± 0.05 and 0.78 ± 0.07/μL respectively.The PCV, Hb, RBC and platelets were significantly higher (P<0.05) in normal lactating compared to that of ovine subclinical mastitis. The MCV, MCH and MCHC showed no significant differences between normal and ewes with subclinical mastitis. While ESR and WBC count were significantly lower in normal lactating.