Abstract
The glutathione S transferase (GST) family is a major part of cellular defense mechanisms against endogenous and exogenous substances, many of which have carcinogenic potential. Alteration in the expression level or structure of the glutathione-S-transferase (GST) enzymes may lead to inadequate detoxification of potential carcinogens and consequently contribute to cancer development. A member of the glutathione-S-transferase (GST) family, GSTP1, is an attractive candidate for involvement in susceptibility to carcinogen-associated colorectal cancer. An A>G transition in exon 5 resulting in an Ile105Val amino acid substitution has been identified which alters catalytic efficiency. The present study investigated the possible impact of Ile105Val GSTP1 polymorphism on susceptibility to colorectal cancer. in Jordan We examined 90 tissue samples previously diagnosed with colorectal carcinoma, and 56 non-cancerous colon tissues. DNA was extracted from paraffin embedded tissues and the status of the GSTP1 polymorphism was determined using a polymerase chain reaction restriction fragment length polymorphism (RFLP) method. No statistically significant differences were found between colorectal cancer cases and controls for the GSTP1 Ile/Ile, Ile/Val and Val/Val genotypes. The glutathione S-transferase polymorphism was not associated with risk in colorectal cancer cases in Jordan stratified by age, sex, site, grade or tumor stage. In conclusion, the GSTP1 Ile105Val polymorphism is unlikely to affect the risk of colorectal cancer.
Highlights
Colorectal cancer (CRC) is the first most common form of cancer affecting Jordanian male population and it accounted for (12.7%) of all newly diagnosed male cancers in 2009
The glutathione S transferase (GST) family is a major part of cellular defense mechanisms against endogenous and exogenous substances, many of which have carcinogenic potential
The present study investigated the possible impact of Ile105Val Glutathione S transferase-P1 (GSTP1) polymorphism on susceptibility to colorectal cancer. in Jordan We examined 90 tissue samples previously diagnosed with colorectal carcinoma, and 56 non-cancerous colon tissues
Summary
Colorectal cancer (CRC) is the first most common form of cancer affecting Jordanian male population and it accounted for (12.7%) of all newly diagnosed male cancers in 2009. Enzymes involved in the detoxification of carcinogenic compounds as well as DNA repair may play a role in the susceptibility to colorectal cancer and other forms of cancer (Vlaykova et al, 2007; Zhang et al, 2007). To date, the potential association between genetic polymorphism of GSTP1 and colorectal carcinoma is somehow still controversial and may vary from population to population (Welfare et al, 1999; Ates et al, 2005; Vlaykova et al, 2007; Epplein et al, 2009; Matakova et al, 2009; Hlavata et al, 2010; Sameer et al, 2012). One method of investigating the protective role of GSTP1 has been to study the effect of polymorphism in GSTP1 gene on susceptibility to colorectal cancer. The main hypotheses investigated in this paper were that polymorphism in the GSTP1 gene predispose to colorectal cancer
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