Abstract
1. 1. Several enzymes of the glucuronic acid pathway were studied in the 7200–12,800 g fraction isolated from the digestive gland of the blue crab. 2. 2. UDPglucose dehydrogenase was observed to reduce NAD when UDPG was the substrate. UDPglucuronic acid acted as substrate for both UDPGApyrophosphatase and UDPGAglucuronyltransferase. EDTA inhibited UDPGA-pyrophosphatase which resulted in increased glucuronide formation. Alkaline phosphatase activity was linear with respect to increased concentration of p- nitrophenyl-β- d- glucuronide . Saccharo-1,4-lactone was observed to have a g great inhibitory effect upon β-glucuronidase. 3. 3. More substrate appears to be utilized via the UDPGA→GA-1-P→GA than UDPGA→glucoronide→GA sequence of reactions because pyrophosphatase activity is greater than transferase activity. 4. 4. It appears, therefore, that the UDPglucose to glucuronic acid part of the glucuronic acid pathway in the blue crab is similar to that reported in mammals.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
