The global strategy employed by Xanthomonas oryzae pv. oryzae to conquer low-oxygen tension

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a notorious rice pathogen that causes bacterial leaf blight (BLB), a destructive rice disease. Low-oxygen tension in the xylem vessels of rice stresses Xoo during infection. In this study, differentially expressed proteins under normoxic and hypoxic conditions were identified using high-performance liquid chromatography (HPLC) coupled with LC-MS/MS to investigate the global effects of low oxygen environment on Xoo PXO99A. A statistically validated list of 187 (normoxia) and 140 (hypoxia) proteins with functional assignments was generated, allowing the reconstruction of central metabolic pathways. Ten proteins involved in aromatic amino acid biosynthesis, glycolysis, butanoate metabolism, propanoate metabolism and biological adhesion were significantly modulated under low-oxygen tension. The genes encoded by these proteins were in-frame deleted, and three of them were determined to be required for full virulence in Xoo. The contributions of these three genes to important virulence-associated functions, including extracellular polysaccharide, cell motility and antioxidative ability, are presented. Biological significanceTo study how Xanthomonas oryzae pv. oryzae (Xoo) conquers low-oxygen tension in the xylem of rice, we identified differentially expressed proteins under normoxic and hypoxia. We found 140 proteins that uniquely expressed under the hypoxia were involved in 33 metabolism pathways. We identified 3 proteins were required for full virulence in Xoo and related to the ability of extracellular polysaccharide, cell motility, and antioxidative. This study is helpful for broadening our knowledge of the metabolism processed of Xoo in the xylem of rice.