Abstract
BackgroundGyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity of Gyejibokryeong-hwan has not yet been well established.MethodsThe present study investigated that the genotoxicity of an aqueous extract of Gyejibokryeong-hwan (GJBRHE): an in vitro chromosomal aberration test using Chinese hamster lung cells, an in vitro bacterial reverse mutation assay (Ames test) with Salmonella typhimurium and Escherichia coli strains, and an in vivo micronucleus test using ICR mouse bone marrow.ResultsGJBRHE with or without the S9 mix showed no genotoxicity in the Ames test up to 5000 μg/plate or in the in vivo MN test up to 2000 mg/kg body weight. In contrast, the chromosomal aberration test showed that GJBRHE induced an increase in the number of chromosomal aberrations compared with the control after treatment for 6 h with 4200 μg/mL GJBRHE in the presence of the S9 mix and for 22 h with 800 μg/mL GJBRHE in the absence of the S9 mix.ConclusionsGJBRHE did not cause detectable genotoxic effects in the bacterial mutation test or the in vivo MN test, however genotoxic effect was detected in the in vitro chromosomal aberration assay. Our results suggest that GJBRHE may be associated with a low risk of carcinogenesis. Thus, further detailed experiments would be needed to clarify the compound responsible for inducing this genotoxicity of GJBRHE and to determine its mechanism.
Highlights
Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome
Quantitative analysis of the seven marker compounds in Gyejibokryeong-hwan The optimized High-performance liquid chromatography (HPLC)–photodiode array (PDA) method was used for quantitative determination of the seven marker compounds in the Gyejibokryeong-hwan sample
The wavelength of the PDA ranged from 190 to 400 nm and the detection wavelengths for quantitative analysis were 262 nm, 230 nm, and 280 nm
Summary
Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. The genotoxicity of Gyejibokryeong-hwan has not yet been well established. Many herbal medicines are widely used to be safety materials than other medicines [1, 2]. Concerns have been raised the scientific evidence for the safety and efficacy of these herbal medicines [5, 6]. The safety information of herbal medicines including oral toxicity, genotoxicity has been not yet enough. A genotoxicity test includes the hazard identification with regard to DNA damage and is required for the development of new drug [7]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have