Genotoxicity evaluation of Hwanglyeonhaedok-tang, an herbal formula

Abstract

Ethnopharmacological relevanceHwanglyeonhaedok-tang (Huanglianjiedu-tang, Orengedoku-to), a traditional herbal formula, is used for the treatment of inflammatory, gastrointestinal and cardiovascular diseases. PurposeThe purpose of this study was to evaluate the genotoxic potential of Hwanglyeonhaedok-tang water extract (HLHDT). MethodsA genotoxicity test was conducted using a bacterial reverse mutation test (Ames test), an in vitro chromosome aberration test using Chinese hamster lung cells, and an in vivo micronucleus test using ICR mouse bone marrow. ResultsIn the Ames test, which used different Salmonella typhimurium (S. typhimurium) and Escherichia coli (E. coli) strains, HLHDT did not increase the number of revertant colonies of S. typhimurium strains TA98, TA100 and TA1535 as well as E. coli strains with or without S9 mix. However, the number of revertant colonies with the S. typhimurium TA1537 strain and S9 mix increased in a dose-dependent manner. The chromosome aberration test showed that HLHDT did not increase the number of structural or numerical chromosome aberrations in a short-period test (6h) with S9 mix. By contrast, HLHDT significantly increased the number of structural chromosome aberrations in a short-period (6h) or continuous (22h) test without S9 mix. In the micronucleus test, no significant increase was observed in micronucleated polychromatic erythrocytes, and no significant decrease was observed in polychromatic to total erythrocytes. ConclusionsThese results indicate that HLHDT might be genotoxic, based on both the Ames and chromosome aberration tests. Therefore, further in vivo studies will be needed to define the mechanism of this genotoxicity.