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Abstract
Major herbaria, such as the one hosted by the botanical garden of Geneva (G) have played a central role in the development of plant systematics over the last 200 years. Today, advances in high throughput sequencing technologies (HTS) together with the development of targeted capture, where DNA extracts are enriched for preselected loci using hybridization probes prior to sequencing, have considerably improved the use of herbaria as a source of genetic data, opening new avenues in the study of plant biodiversity.
Highlights
Since 2016, research conducted at Conservatory and Botanical Garden of Geneva using high throughput sequencing technologies (HTS) approaches on herbarium specimens were mainly focused on three taxonomical groups
The genus Silene in the Caryophylaceae family was investigated with the aim of defining the relationship and species boundaries in the section Italicae
The Sapotaceae family was investigated with the aim of refining generic and species circumscriptions in the family
Summary
Since 2016, research conducted at Conservatory and Botanical Garden of Geneva using HTS approaches on herbarium specimens were mainly focused on three taxonomical groups. Specimens were mainly from the Mediterranean region, with a total of 133 samples, with 56 % of herbarium origin (oldest 1813, mean 1970, SD 37.4 years). The kit used targeted 256 regions for a total of 650 000 bp.
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