Abstract
The genome-wide transcriptional response to 20-hydroxyecdisone at the onset of Drosophila metamorphosis, as well as its dependency on one of the ecdysone receptors is described.
Highlights
The steroid hormone 20-hydroxyecdysone (20E) triggers the major developmental transitions in Drosophila, including molting and metamorphosis, and provides a model system for defining the developmental and molecular mechanisms of steroid signaling. 20E acts via a heterodimer of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspiracle, to directly regulate target gene transcription
We examine the effects of disrupting EcR function on the global patterns of gene expression at the onset of metamorphosis, and use these data to refine our lists of 20E-regulated genes
This comparison demonstrates that the microarrays accurately reflect the temporal patterns of 20E-regulated gene expression at the onset of metamorphosis and have sufficient sensitivity to detect rare transcripts such as EcR and E74A
Summary
The steroid hormone 20-hydroxyecdysone (20E) triggers the major developmental transitions in Drosophila, including molting and metamorphosis, and provides a model system for defining the developmental and molecular mechanisms of steroid signaling. 20E acts via a heterodimer of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspiracle, to directly regulate target gene transcription. The steroid hormone 20-hydroxyecdysone (20E) triggers the major developmental transitions in Drosophila, including molting and metamorphosis, and provides a model system for defining the developmental and molecular mechanisms of steroid signaling. 20E acts via a heterodimer of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspiracle, to directly regulate target gene transcription Small lipophilic hormones such as retinoic acid, thyroid hormone, and steroids control a wide range of biological pathways in higher organisms. These hormonal signals are transduced into changes in gene expression by members of the nuclear receptor superfamily that act as hormone-responsive transcription factors [1]. Pulses of the steroid hormone 20-hydroxyecdysone (20E) act as critical temporal signals that direct each of the major developmental transitions in the Drosophila life cycle, including molting and metamorphosis [2].
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