Abstract
Spinal cord injury (SCI) is a severe complication of acute spinal injury, it can lead to axonal degeneration and necrosis of neurons, causing damage cross section below the movement, then loss of sensory, reflex and autonomous control function below the damage section. Nowadays, there is increasing evidence that transplantation of neural stem cells can help to repair spinal cord injury after spinal cord injury. The aim of this study was to research the function of miR-31 expression in differentiation of neural stem cells. Mir-31 was transfected the mimics and inhibitor into neural stem cells. By the morphological observation, the cell over expression of miR-31 was closer to NSCs. With the RT-PCR, Hb9 and STMN1 were up-regulate when miR-31 was inhibited, and Nestin was down-regulate. When miR-31 was over expression, the expression of Nestin and Hb9 was up-regulate. These findings suggest that miR-31 may play a significant role in proliferation and differentiation of neural stem cell and are potential targets for therapeutic interventions following spinal cord injury.
Highlights
There is a wide variety of reasons happened to spinal cord injury (SCI)
Recent researches have shown that nerve stem cells (NSCs) replacement is an effective strategy for the treatment of SCI. (Paul Lu et al, 2014; Suzuki SO et al, 2003) [1] [2]
MicroRNAs play an important role in gene regulated, it binds to complementary sites on the 3’-untranslated regions of the target mRNAs to induce cleavage or repression of translation
Summary
There is a wide variety of reasons happened to spinal cord injury (SCI). The most common cause for spinal cord is incident trauma. The clinical treatment for SCI lacks effective and safe strategies which have become a severe socioeconomically issue. Recent researches have shown that nerve stem cells (NSCs) replacement is an effective strategy for the treatment of SCI. (Paul Lu et al, 2014; Suzuki SO et al, 2003) [1] [2]. How induce more NSCs to motor neuron cells (MNs) is the vital part. MicroRNAs are a novel class of short, 18 - 25 nucleotide-long non-coding small RNAs. MicroRNAs are a novel class of short, 18 - 25 nucleotide-long non-coding small RNAs It play an important regulatory role in gene expression by binding to the 3’untranslated region of target mRNAs transcripts for de-
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