The frog taste disc: A prototype of the vertebrate gustatory organ

Abstract

The frog taste disc (TD) is apparently the largest gustatory organ found in vertebrates and seems to differentiate into a specialized variety of the prototypic scheme of the taste bud. An explanation for this unusual organization is lacking although it is possible to speculate the existence of environmental and nutritional requirements. Up to the present time, the most common model of the D main cell types (sensory and sustentacular). This model may oversimplify the morphology of this structure since more numerous cell types have been described. We now propose a new model of the TD, based on comprehensive data on the ultrastructure of the organ obtained in the last 20 years. The main conclusions are the following: (1) the TD is a pluristratified epithelium with a general organization similar to that of the olfactory and vomeronasal epithelium; (2) it has a skeleton composed of three different types of epithelial cells; (3) the chemoreceptorial surface is covered by different microenvironment; (4) three different types of neuro-epithelial systems are present; the type II is an ‘open’ sensory cell with axonal contacts devoid of vesicles; the type III is an ‘open’ sensory cell with synaptic-like junctions; the type IV is a ‘closed’ sensory cell with a ‘Merkel-neurite complex’; (5) the nerve fibers in the basal plexus are mostly cholinergic while the peridiscal nerve fibers are mostly peptidergic. The presence of several cell types in the TD must be considered using these large receptors in electrophysiological studies or as a source of isolated cells, and their complexity must induce caution in the interpretation of the data. Text books of histology usually describe the peripheral structures associated with taste as very simple: an idea that probably must be revised. A taste organ is a highly complex structure composed of several sensory systems and a comparative approach can aid comprehension of its general organization. The study of the ‘large taste organs’ present in some species of amphibians can provide useful data for knowledge of the gustatory system of vertebrates.