Abstract
Method A cross-sectional analysis of immune and viral parameters was performed on 44 HIV-infected children (median 7.8 yrs, 0.08-18.6) that were untreated at time of biological assessment. Frequency of IFN-γ producing CD8 lymphocytes in response to Env-Gag-Pol antigens was determined using the ELISPOT technique. HIV-RNA level was determined by PCR (monitor 1.5, Roche). HIV DNA level was determined in total PBMC, using real time PCR in LTR (ANRS method). Associations between quantitative variables were defined by Pearson's partial correlations. Partial correlation measures the strength of relationship between two variables, controlling for the effect of one or more other variables.
Highlights
Open AccessFlorence Buseyne*1, Stéphane Blanche, Marianne Burgard, Catherine Milliancourt, Daniel Scott-Algara, Christine Rouzioux and
HIV infection during the perinatal period differs from infection during adulthood by clinical progression, dynamic of viral replication, level of thymic activity and maturity of the immune system at time of infection.HIV-DNA level in PBMC has recently been shown to be an independent predictor of disease progression
We investigated whether any association could be found between HIV-DNA load in PBMC and the HIV-specific CD8 T lymphocytes frequency
Summary
Florence Buseyne*1, Stéphane Blanche, Marianne Burgard, Catherine Milliancourt, Daniel Scott-Algara, Christine Rouzioux and. Address: 1Unité postulante d'Immunopathologie Virale, Institut Pasteur, Paris, France, 2Fédération de Pédiatrie, Hôpital Necker, Paris, France, 3Laboratoire de Virologie, EA3620 Université René Descartes, Hôpital Necker, Paris, France and 4Unité de Régulation des Infections Rétrovirales, Institut Pasteur, Paris, France. Published: 9 April 2008 Retrovirology 2008, 5(Suppl 1):O22 doi:10.1186/1742-4690-5-S1-O22. Maternal chronic viral infections transmitted to infants: from mechanisms to prevention and care Meeting abstracts - A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1742-4690-5-S1-info.pdf
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