Abstract
Samples of saliva from 16 secretors and 12 non-secretors have been fractionated by gel permeation chromatography on Sephadex G100 and the fractions tested for blood group activity using an auto-analyser. In contrast to previously reported work we detected only one blood group active component of high molecular weight in the eluate, and no further fractionation of saliva into lower molecular weight substances possessing blood group activity was found. Similar results were obtained when a range of anti-sera were studied. Attention is drawn to anomalous results that may be produced during GPC of protein-carbohydrate complexes as found in saliva due to adsorption or precipitation on the column.
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