Abstract
Naturally occurring dissolved humic materials were fractionated according to molecular weight by dialysis and gel permeation chromatography. Components of higher molecular weight (>3,500) absorbed strongly at 250 nm but fluoresced weakly, while humic fractions of lower molecular weight fluoresced intensely per unit absorbance. With appropriate precautions, fluorescence: absorbance ratios may be useful in delineating seasonal and depth distribution patterns of dissolved humic materials of low and high molecular weight.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have