Abstract
We found a novel dystrophin-associated protein (DAP) exhibiting almost the same mobility as γ-sarcoglycan on SDS-PAGE. This novel DAP with basic charge is separated from γ-sarcoglycan by 2-dimensional PAGE or de- N-glycosylation followed by SDS-PAGE. This DAP is most likely the rabbit homologue of “δ-sarcoglycan”, the γ-sarcoglycan-like protein identified previously [Nigro et al. (1996) Hum. Mol. Genet. 5, 1179–1186], since an internal amino acid sequence from the DAP matched the predicted amino acid sequence of “human δ-sarcoglycan” within the limits of species difference and this DAP was recognized by anti-“δ-sarcoglycan” antibody. The DAP was found to be contained in the sarcoglycan fraction which was prepared by treatment of the dystrophin-DAP complex with n-octyl β- d-glucoside and crosslinked with β- and/or γ-sarcoglycan by a chemical crosslinker, dithiobis(succinimidyl propionate). Therefore, we concluded that the DAP is the fourth component of the sarcoglycan complex. © 1997 Federation of European Biochemical Societies.
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