The [formula omitted] mutation: Nucleotide sequence and comparative analysis

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Nucleotide sequence analysis of mRNA from the class I murine MHC mutant H-2Kkml has established a site of mutation to be at the codon for amino acid position 152. Complete sequence information for the nucleotides coding for amino acids 136–163 demonstrates an A → C alteration at the codon for amino acid 152, changing Asp (GAT) in Kk to Ala (GCT) in Kkml . Several other murine and human class I MHC variants have similar alterations at amino acid position 152, resulting in altered biological activity. Finally, the pH-2III pseudogene of the H-2k haplotype has a GCT codon at amino acid position 152, suggesting that the GCT codon occurring in Kkml is the result of a gene conversion event.