Abstract
We present the first genetic map of tedera (Bituminaria bituminosa (L.) C.H. Stirton), a drought-tolerant forage legume from the Canary Islands with useful pharmaceutical properties. It is also the first genetic map for any species in the tribe Psoraleeae (Fabaceae). The map comprises 2042 genotyping-by-sequencing (GBS) markers distributed across 10 linkage groups, consistent with the haploid chromosome count for this species (n = 10). Sequence tags from the markers were used to find homologous matches in the genome sequences of the closely related species in the Phaseoleae tribe: soybean, common bean, and cowpea. No tedera linkage groups align in their entirety to chromosomes in any of these phaseoloid species, but there are long stretches of collinearity that could be used in tedera research for gene discovery purposes using the better-resourced phaseoloid species. Using Ks analysis of a tedera transcriptome against five legume genomes provides an estimated divergence time of 17.4 million years between tedera and soybean. Genomic information and resources developed here will be invaluable for breeding tedera varieties for forage and pharmaceutical purposes.
Highlights
The Psoraleeae is a tribe of legumes (Fabaceae) distributed across Africa, Europe, Australia and the Americas [1]
We describe a modified Double Digest RADseq (ddRAD) method incorporating variable length inline barcodes, which allows considerable control over tag number and produces highly diverse libraries throughout sequencing cycles. The objective of this project was to develop a genetic map of the tedera genome, the first for this species and the first for any member of the legume tribe Psoraleeae
Alignments of the tedera genetic map with the genomes of soybean, common bean and cowpea (Figure 2; Figure 3) indicates that genomic information in these well-studied crops will be relatively straightforward to leverage in tedera
Summary
The Psoraleeae is a tribe of legumes (Fabaceae) distributed across Africa, Europe, Australia and the Americas [1]. It includes economically significant species used in traditional agriculture in North. America (Pediomelum esculentum, Indian breadroot) [1] and the Canary Islands and Mediterranean. Var. bituminosa is widely distributed in the Mediterranean basin and Macaronesia. It is a self-pollinated species [5] with low levels of outcrossing (
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call