Abstract
Electron spin resonance (ESR) spectroscopy was used to investigate the generation of free radicals under pulsed electric field (PEF) processing. Spin trapping studies, using 5,5-dimethyl-1-pyrroline N-oxide (DMPO), showed the generation of hydrogen radicals in phosphate buffer and oleic acid emulsion. The concentration of hydrogen peroxide in phosphate buffer after PEF treatment was determined by spectrofluorometric method. Electric field strength has a significant effect on the concentration of H 2O 2 ( p < 0.05). In PEF-treated samples with electric field strength of 30 kV/cm and 35 kV/cm, trace concentrations of H 2O 2 (0.177 × 10 −6 and 2.035 × 10 −6 mol/L, respectively) were detected.
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