Abstract
A procedure for the isolation of aleurone grains from seeds of Vicia faba is described which gives a preparation essentially free of starch grains, nuclei, mitochondria and ribosomes. The stability of the grains was studied over a range of concentrations of sucrose, phosphate and magnesium chloride. The isolated grains were found to contain 14·1 per cent by weight of non-dialysable nitrogen and 1·4 per cent by weight of phosphorus, the majority of which was phytate. No convincing evidence was obtained to suggest that the grains contain RNA. The major proteins present in the grains were the storage proteins, vicilin and legumin, but in addition to these were several different albumins. The albumins of aleurone grains isolated from 24-hr soaked seeds possessed proteolytic activity and acid phosphatase activities. Phytase activity, however, was only shown by albumins isolated from aleurone grains of seeds germinated for periods longer than 24 hr.
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