Abstract
Cotyledonary cells of mature Vicia faba seeds contain large amounts of protein bodies. Beside the lobed nucleus a few mitochondria and starch grains are present as well as some spherosomes around the amyloplasts and along the plasmalemma. Protein bodies were isolated in different aqueous and nonaqueous media. The yield and purity of resulting organelle fractions after the isolation procedures were estimated by quantitative serological and chemical determinations as well as by electron microscopy. The contents of protein bodies exhibit the same globulin patterns compared with total salt extracts of seed flour as revealed by polyacrylamide gelelectrophoresis and two-dimensional immunoelectrophoresis. Quantitation of phytohemagglutinin by serological techniques in protein body extracts gave amounts of about 1%. The Vicia faba lectin was absent in other organs and starch grains but present in cotyledons. E dax analysis of elements in connections with scanning electron microscopy revealed the presence of phosphate, potassium, sulphur, and calcium within the protein bodies.
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