The expressions of CSE/H2S and RvE1 in the patients with ulcerative colitis

Abstract

Objective To investigate the expressions of endogenous cystathionine-γ-lyase (CSE)/hydrogen sulfide (H2S) and resolvin E1 (RvE1) in patients with ulcerative colitis (UC), and its effect on the pathogenesis of ulcerative colitis. Methods The distribution and expression of CSE proteins in the rectum mucosa in 60 cases of UC and 30 cases of normal control group were detected by Strept Avidin-Biotin Complex (SABC) immunohistochemistry. The average optical density value of CSE was analyzed with an Image Analyzing systems. The expression of CSE mRNA in the rectum mucosa was detected by real-time polymerase chain reaction (RT-PCR). The levels of H2S and RvE1 in sera were detected by spectrophotometry. Results The expressions of CSE proteins in three groups were detected in the rectum mucosa membrane epithelia. The average optical density value of CSE and the expression of CSE mRNA in patients with active UC were higher than that in normal group and remission of UC. The levels of H2S and RvE1 in patients with active UC were significantly higher than that in normal group and remission of UC. Conclusions The abnormal expressions of CSE/H2S and RvE1 in activity of the UC might play an important role in the pathogenesis of UC. Key words: Cystathionine gamma-lyase/ME; Hydrogen sulfide/ME; Fatty acids, unsaturated/ME; Colitis, ulcerative/ME