Expression of endogenous heme oxygenase-1/carbon monoxide system and resolvin E1 in the patients of Crohn disease

Abstract

Objective To investigated the change of endogenous heme oxygenase-1 (HO-1)/carbon monoxide (CO) system and resolvin E1(RvE1) in patients with crohn disease (CD), and its effects on the pathogenesis of ulcerative colitis. Methods The distribution and expression of HO-1 proteins in the colonic mucous in 40 cases with CD and 15 cases of normal control group were detected by SABC immunohistochemistry. The average optical density value of HO-1 was analyzed with an Image Analyzing systems. The concentration of CO in the colonic mucosa was detected with chemical methods. The levels of RvE1 in serum was detected by enzyme linked immunosorbent assay (ELISA). Results The expression of HO-1 proteins in patients with active CD were significantly higherin the colonic mucous membrane epithelia. The average optical density value of HO-1 in patients with active CD was higher than that in normal group and remission of CD(0.358 ± 0.021 vs. 0.163 ± 0.018 and 0.177 ± 0.032)(P < 0.01). The concentrations of CO in active CD was significantly higher than that in the normal control group and remission of CD [(23.79 ± 1.81) μmol/g vs. (18.68 ± 1.64) and (17.13 ± 1.23) μmol/g](P < 0.01). The levels of RvE1 in patients with active CD were significantly higher than those in normal group and remission of CD [(41.35 ± 2.76) ng/ml vs. (15.34 ± 2.19) and (14.51 ± 1.47) ng/ml](P < 0.01). Conclusions The abnormal expression of HO-1/CO system and RvE in activity of the CD may play an important role in the pathogenesis of CD. Key words: Heme oxygenase-1; Carbon monoxide; Resolvin E1; Crohn disease