The expressions of brain-derived neurotrophic factor and nerve growth factor in purified rat choroid plexus epithelial cells in vitro

Abstract

ObjectiveTo detect the expressions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in purified rat choroid plexus epithelial cells in vitro. MethodsPrimary and passage choroid plexus epithelial cells were obtained from newborn, one-day Sprague-Dawley rats. The expressions of BDNF and NGF were measured by qRT-PCR and Western blottingting. The secretions of BDNF and NGF were detected by ELISA. Cell supernatants of primary cells, purified cells and passage 1 cells were harvested. ResultsThe expression of BDNF in the purified cells was significantly lower than that in the primary cells (P<0.05), and it in the primary cells and the purified cells was significantly higher than that in the passage 1 cells (P<0.05). The expression of NGF was significantly higher in the purified cells than in the primary cells and the passage 1 cells (P<0.05). It in the passage 1 cells was significantly higher than that in the primary cells (P<0.05). ConclusionThe time of CPECs transplantation for central nervous system diseases should be selected based on their secretory function and features, which could lead to better and more effective treatment.