Abstract
PLIN2 (Perilipin-2) is a protein that can anchor on the membrane of lipid droplets (LDs), playing a vital role in the early formation of LDs and in the regulation of LD metabolism in many types of cells. However, little research has been conducted in cattle adipocytes. In the present study, we found that the expression of PLIN2 mRNA peaks at Day 2 during cattle adipocyte differentiation (p < 0.01), but PLIN2 protein levels maintain high abundance until Day 4 and then decrease sharply. We first built an interaction model using PyMOL. The results of a pull-down assay indicated that bovine PLIN2 and CGI-58 (ABHD5, α/β hydrolase domain-containing protein 5) had an interaction relationship. Furthermore, Bimolecular Fluorescence Complementation-Flow Cytometry (BiFC-FC) was used to explore the function of the PLIN2-CGI-58 interaction. Interestingly, we found that different combined models had different levels of fluorescence intensity; specifically, PLIN2-VN173+CGI-58-VC155 expressed in bovine adipocytes exhibited the highest level of fluorescence intensity. Our findings elucidate the PLIN2 expression pattern in cattle adipocytes, the protein structure and the function of protein–protein interactions (PPI) as well as highlight the characteristics of bovine PLIN2 during the early formation and accumulation of lipid droplets.
Highlights
Lipid droplets (LD), which are generally called vesicles, have a structure similar to small balloons that are full of neutral lipids and anchored by many different proteins [1,2]
An LD is surrounded by a single layer of phospholipid molecules [6], the core components of which consist of hydrophobic neutral fats (primarily ATGL and cholesterol ester) that are maintained on the surface with specially structured anchor proteins [7]
Studies on 3T3-L1 murine adipocytes showed the preferential association of PLIN2 with lipid droplets compared with PLIN1, implying that PLIN2 is more important than PLIN1 in lipid formation [13], which indicates that PLIN2 can be used as a marker gene for precursors during adipocyte differentiation
Summary
Lipid droplets (LD), which are generally called vesicles, have a structure similar to small balloons that are full of neutral lipids and anchored by many different proteins [1,2]. A recent study showed that PLIN2 is a type of factor primarily expressed in the early process of adipocyte differentiation and promotes fat droplet formation in muscle cells [11]. Studies in mice have shown that PLIN2 is usually located on small droplets [15] These results lead us to question whether there are different expression patterns for the gene and the protein in live stocks, what the structure of this protein is, and whether this protein could be involved in interactions with other proteins. Based on its role in adipogenesis, which was demonstrated in mice, we propose the hypothesis that similar variations in the PLIN2 mRNA and protein expression pattern exist in cattle. 2017, 18, x FOR PEER REVIEW the first to identify PLIN2 expression in cattle adipocytes and to characterize an interaction function 2.12..1S.eqSuieneqnuvcietenroHceaoHnmdoomilnoogvlyoi,gvIyon,.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
