Abstract
Objective To observe the expression of transforming growth factor-β1 (TGF-β1) dur-ing the healing of rats with diabetes and explore its influence on delayed union. Methods Seventy rats were divided randomly into experimental group and control group. Streptozotocin (60 mg/kg) was injected into the enterocoelia of experimental group to induce diabetes model (the blood sugar level > 11.2 mmol/ L). Nomal saline was given instead to the control group. All animals suffered left tibia fracture. Regular X-ray photographs were taken, bony callus was subjected to HE staining and the levels of TGF-β1 in the bony callus and serum were detected by immunohistochemical method and enzyme-linked immunosorbent assay (ELISA) respectively. Results As compared with the control group, the bone formation was signifi-cantly delayed in the experimental group at the 1st, 2nd, 3rd, 4th, 6th, 8th week both from X-ray and HE sections. Immunohistochemical staining and ELISA revealed that there was no significant difference in the levels of TGF-β1 between two groups at the 3rd week. The gray scales at the 3rd week were 189. 59±2.76 in diabetes group and 166.74 ± 1.33 in control group (P < 0. 05 ), respectively. The serum levels at the 3rd week were (12. 05 ± 1.56) μg/L in diabetes group and (17.48±0. 56) μg/L in control group (P < 0. 05 ), respectively. Conclusion The decrease of TGF-β1 in both serum and bony callus is one of causes for poor fracture healing in rats with diabetes after fracture. Key words: Transforming growth factor; Diabetes; Fracture; Delayed union
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
